DIFFERENTIATION OF HELICOBACTER SPECIES BY NUMERICAL-ANALYSIS OF THEIR ONE-DIMENSIONAL ELECTROPHORETIC PROTEIN-PATTERNS

Citation
M. Costas et al., DIFFERENTIATION OF HELICOBACTER SPECIES BY NUMERICAL-ANALYSIS OF THEIR ONE-DIMENSIONAL ELECTROPHORETIC PROTEIN-PATTERNS, Systematic and applied microbiology, 16(3), 1993, pp. 396-404
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
0723-2020
Volume
16
Issue
3
Year of publication
1993
Pages
396 - 404
Database
ISI
SICI code
0723-2020(1993)16:3<396:DOHSBN>2.0.ZU;2-V
Abstract
The suitability of sodium dodecyl sulfate-polyacrylamide gel electroph oretic protein profiles coupled to a computer-assisted numerical analy sis for the differentiation of helicobacters was evaluated. Fifty-thre e strains representing most of the known and available species of Heli cobacter and reference strains of allied taxa were included. The resul ts indicate, (i) that the majority of the species examined by this tec hnique could be clearly differentiated, including H. cinaedi and H. fe nnelliae, which can be difficult to separate by conventional bacteriol ogical methods; two species, H. acinonyx and H. pylori constitute a si ngle phenon at the 69% similarity level and cannot be distinguished at this level, (ii) that gastric helicobacters were more closely cluster ed to each other than they mere to those of enteric origin, (iii) that within several species, different electrophoretic types could be dist inguished and that, in the case of the H. pylori/H. acinonyx complex, there appeared to be clustering based on the host source of the strain s. Two minor groups (one of human and one of canine origin) were ident ified and these may merit further phylogenetic analysis. The suitabili ty of sodium dodecyl sulfate-polyacrylamide gel electrophoretic protei n profiles coupled to a computer-assisted numerical analysis for the d ifferentiation of helicobacters was evaluated. Fifty-three strains rep resenting most of the known and available species of Helicobacter and reference strains of allied taxa were included. The results indicate, (i) that the majority of the species examined by this technique could be clearly differentiated, including H. cinaedi and H. fennelliae, whi ch can be difficult to separate by conventional bacteriological method s; two species, H. acinonyx and H. pylori constitute a single phenon a t the 69% similarity level and cannot be distinguished at this level, (ii) that gastric helicobacters were more closely clustered to each ot her than they mere to those of enteric origin, (iii) that within sever al species, different electrophoretic types could be distinguished and that, in the case of the H. pylori/H. acinonyx complex, there appeare d to be clustering based on the host source of the strains. Two minor groups (one of human and one of canine origin) were identified and the se may merit further phylogenetic analysis.