TAXONOMIC SIGNIFICANCE OF THE DISTRIBUTION OF COMPONENT PARTS OF POLAR ETHER LIPIDS IN METHANOGENS

Citation
Y. Koga et al., TAXONOMIC SIGNIFICANCE OF THE DISTRIBUTION OF COMPONENT PARTS OF POLAR ETHER LIPIDS IN METHANOGENS, Systematic and applied microbiology, 16(3), 1993, pp. 342-351
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
0723-2020
Volume
16
Issue
3
Year of publication
1993
Pages
342 - 351
Database
ISI
SICI code
0723-2020(1993)16:3<342:TSOTDO>2.0.ZU;2-7
Abstract
The ether core lipids, phospholipid-polar head groups and glycolipid-s ugar moieties (component parts of polar lipids) from 18 species of met hanogens, belonging to 5 families, were analysed qualitatively at the total lipid level, without separation of lipids after extraction, and the results were cumulated to the data published previously from 13 ot her species. Archaeol, caldarchaeol, macrocyclic archaeol, and two kin ds of hydroxyarchaeol were found as core lipids; myo-inositol, ethanol amine, serine, aminopentanetetrols, glycerol, and N-acetylglucosamine were identified as phospholipid-polar head groups; and glucose, galact ose, and mannose as glycolipid-sugars in methanogens as a whole. The d istribution of these component parts, regardless their arrangement in the lipid molecules, was characteristic of methanogen taxonomic groups at a family or genus level, and, therefore, coincided with the classi fication based on the 16S rRNA analysis. This shows that lipid compone nt parts could become a new chemotaxonomic marker, which utilises more lipid-structure-oriented information than a thin-layer chromatographi c pattern. A determinative key of methanogens, only based on lipid com ponent analysis, is proposed.