TISSUE DISTRIBUTION OF CYTOCHROME-C-OXIDASE ISOFORMS IN MAMMALS - CHARACTERIZATION WITH MONOCLONAL AND POLYCLONAL ANTIBODIES

Citation
Jw. Taanman et al., TISSUE DISTRIBUTION OF CYTOCHROME-C-OXIDASE ISOFORMS IN MAMMALS - CHARACTERIZATION WITH MONOCLONAL AND POLYCLONAL ANTIBODIES, Biochimica et biophysica acta, 1225(1), 1993, pp. 95-100
Citations number
40
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biophysics,Biology
ISSN journal
0006-3002
Volume
1225
Issue
1
Year of publication
1993
Pages
95 - 100
Database
ISI
SICI code
0006-3002(1993)1225:1<95:TDOCII>2.0.ZU;2-6
Abstract
Monoclonal and polyclonal antibodies specific to the two isoforms of s ubunit VIa of bovine cytochrome c oxidase were generated and used to s tudy the tissue distribution of this subunit pair in beef, human and r at. The so-called H-(heart) form was found exclusively in heart and sk eletal muscle, whereas the so-called L-(liver) form was the only isofo rm present in brain, kidney, liver and smooth muscle. Little or no L-f orm was detected in skeletal muscle. In bovine heart no subunit VIa-L was detected, while in human heart the subunit VIa-H and VIa-L isoform s were present in roughly equal proportions. These results imply that, in humans, the deficiency of a subunit VIa isoform may have a differe nt effect on the physiology of heart then on the physiology of skeleta l muscle.