PLASMA-MEMBRANE RECYCLING IN CFTR-EXPRESSING CHO CELLS

Citation
S. Dho et al., PLASMA-MEMBRANE RECYCLING IN CFTR-EXPRESSING CHO CELLS, Biochimica et biophysica acta, 1225(1), 1993, pp. 78-82
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biophysics,Biology
ISSN journal
0006-3002
Volume
1225
Issue
1
Year of publication
1993
Pages
78 - 82
Database
ISI
SICI code
0006-3002(1993)1225:1<78:PRICCC>2.0.ZU;2-1
Abstract
The hypothesis that the cystic fibrosis transmembrane conductance regu lator (CFTR) participates in plasma membrane recycling was tested expe rimentally. Using CHO cells, we determined the effects of CFTR express ion and of elevated intracellular cAMP on exocytosis, measured as the incorporation into the plasma membrane of endosomes pre-labelled with biotinylated wheat-germ agglutinin (WGA). CFTR expression was without effect on the rate of exocytosis. Furthermore, cAMP did not affect end osomal recycling to the plasma membrane in either CFTR-expressing or c ontrol cells. These findings suggest that CFTR is not involved in regu lating plasma membrane recycling in all cells.