THE HUMAN GCAP1 AND GCAP2 GENES ARE ARRANGED IN A TAIL-TO-TAIL ARRAY ON THE SHORT ARM OF CHROMOSOME-6 (P21.1)

Citation
A. Surguchov et al., THE HUMAN GCAP1 AND GCAP2 GENES ARE ARRANGED IN A TAIL-TO-TAIL ARRAY ON THE SHORT ARM OF CHROMOSOME-6 (P21.1), Genomics, 39(3), 1997, pp. 312-322
Citations number
42
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Genetics & Heredity
Journal title
ISSN journal
0888-7543
Volume
39
Issue
3
Year of publication
1997
Pages
312 - 322
Database
ISI
SICI code
0888-7543(1997)39:3<312:THGAGG>2.0.ZU;2-O
Abstract
GCAP1 and GCAP2 are related Ca2+-binding proteins that activate photor eceptor guanylate cyclase(s). We showed previously that the human GCAP 1 gene, consisting of four exons, is located at 6p21.1 (locus designat ion GUCA). To identify the chromosomal location of the GCAP2 gene, we first cloned its cDNA and determined its intron-exon distribution by P CR analysis. The results show that the introns of the GCAP2 gene are p ositioned exactly as in the GCAP1 gene and are nearly double in size. Sequence similarity between the two genes, however, is limited to port ions of exons 1 and 2. The GCAP1 and GCAP2 genes are transcribed into single mRNA species (1.7 and 2.2 kb, respectively) and are detectable only in the retina by Northern blotting. The GCAP2 gene was found by s omatic human-hamster hybrid panel analysis and FISH to reside at GUCA in a region indistinguishable from that of GCAP1. PCR analysis with ex on 4-specific primers showed that the genes are in a tail-to-tail arra y less than 5 kb apart and altogether span less than 20 kb of genomic DNA. The identical gene structures and loci of GCAP1 and GCAP2, and th e identical function of the gene products, are consistent with a gene duplication event. (C) 1997 Academic Press.