TREATMENT OF FISH PARASITES .10. EFFECTS OF A NEW TRIAZINE DERIVATIVE, HOE-092-V, ON MONOGENEA - A LIGHT AND TRANSMISSION ELECTRON-MICROSCOPY STUDY

Authors
Citation
G. Schmahl, TREATMENT OF FISH PARASITES .10. EFFECTS OF A NEW TRIAZINE DERIVATIVE, HOE-092-V, ON MONOGENEA - A LIGHT AND TRANSMISSION ELECTRON-MICROSCOPY STUDY, Parasitology research, 79(7), 1993, pp. 559-566
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Parasitiology
Journal title
ISSN journal
0932-0113
Volume
79
Issue
7
Year of publication
1993
Pages
559 - 566
Database
ISI
SICI code
0932-0113(1993)79:7<559:TOFP.E>2.0.ZU;2-7
Abstract
For chemotherapy of fish parasitized by monogeneans, a novel triazine derivative, phenyl]-1-methyl-hexahydro-1,2,4-triazine-3,5-dion (HOE 09 2 V), was tested in vivo against the gill- and skin-parasitizing speci es Dactylogyrus extensus, D. vastator, and Gyrodactylus arcuatus. Natu rally infected fish were incubated in aerated, separate tanks at 22-de grees-C for 1, 2, 3, and 4 h in water containing 0, 1, 5, 10, or 15 mu g HOE 092 V/ml, whereas Pseudodactylogyrus bini was tested in vitro at 10 mug HOE 092 V/ml for 2.25 h. As seen by means of transmission elec tron microscopy, in vivo treatment against D. extensus caused vacuoliz ation and lysis of the parasite's tegument at a dose as low as 1 mug/m l over a 3-h exposure period. Higher doses, such as 5 and 10 mug/ml ov er the same exposure period, produced lesions in the circular and long itudinal musculature of D. extensus and differing degrees of damage to the ciliary cells of protonephridia and immature vitelline cells. The re was 100% mortality in D. vastator when incubation was done with 10 mug HOE 092 V/ml for 4 h (G. arcuatus: 5 mug/ml for 4 h; 10 mug/ml for 1 h) and in P. bini after 2.25 h in vitro exposure. In all species te sted, the anterior portion and the opisthaptor region were most sensit ive to the drug action. This study shows that fish infected with Gyrod actylus spp., Dactylogyrus spp., and/or Pseudodactylogyrus spp. can be treated successfully in a water bath containing 10 mug HOE 092 V/ml.