ALLOGENIC BMT IN CHILDREN - DIFFERENTIAL LYMPHOCYTE SUBSET RECONSTITUTION ACCORDING TO THE OCCURRENCE OF ACUTE GVHD

Citation
L. Garin et al., ALLOGENIC BMT IN CHILDREN - DIFFERENTIAL LYMPHOCYTE SUBSET RECONSTITUTION ACCORDING TO THE OCCURRENCE OF ACUTE GVHD, Clinical immunology and immunopathology, 77(2), 1995, pp. 139-148
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pathology,Immunology
ISSN journal
0090-1229
Volume
77
Issue
2
Year of publication
1995
Pages
139 - 148
Database
ISI
SICI code
0090-1229(1995)77:2<139:ABIC-D>2.0.ZU;2-M
Abstract
To acquire some biological markers associated with the occurrence of a cute graft-versus-host disease (aGVHD) after allogeneic bone marrow tr ansplant (BMT) in children, we have studied the lymphocyte subset reco nstitution and the percentage of peripheral blood mononuclear cells be aring HLA-DR and HLA-DQ class II molecules, This study included 37 all ogeneic BMT: either with (n = 17) or without (n = 20) aGVHD. Within 2 months after transplantation, we observed that patients with aGVHD had a unique mononuclear cell profile characterized by (i) a significant increase in the percentages of CD8(bright+)CD28(-) T cells (P = 0.05) and CD3(+) T cells (P = 0.001), (ii) an important decrease in the perc entage of CD56(+) cells (P = 0.0001), and (iii) a decrease in the perc entages of HLA-DQ(+) and HLA-DR(+) monocytes (P = 0.001), and HLA-DQ() T lymphocytes (P = 0.0001), in comparison with patients without aGVH D. Moreover, statistical studies indicate that there was a positive co rrelation between CD8(bright)CCD28(-) and CD3(+) T cells, whereas CD3( +) T cells were negatively correlated to CD56(+) cells. We did not fin d any statistical correlation between the percentages of HLA-DQ(+) or HLA-DR(+) cells and the percentages of these lymphocyte subsets, There fore, in this study done in children, we suggest that patients with (i ) less than 20% of DQ(+) monocytes, (ii) less than 25% of CD56(+) lymp hocytes, and (iii) an enhanced percentage of CD8(bright)CCD28(-) T cel ls are strongly associated with aGVHD. Unfortunately, these biological markers of aCVHD may not precede the clinical manifestations of the d isease. (C) 1995 Academic Press, Inc.