THE FATE OF MYOBLASTS FOLLOWING TRANSPLANTATION INTO MATURE MUSCLE

Citation
Ta. Rando et al., THE FATE OF MYOBLASTS FOLLOWING TRANSPLANTATION INTO MATURE MUSCLE, Experimental cell research, 220(2), 1995, pp. 383-389
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology,"Cell Biology
Journal title
ISSN journal
0014-4827
Volume
220
Issue
2
Year of publication
1995
Pages
383 - 389
Database
ISI
SICI code
0014-4827(1995)220:2<383:TFOMFT>2.0.ZU;2-T
Abstract
Cell transplantation has potential benefits for tissue replacement in the the enhancement of tissue regeneration and as cell-mediated gene t herapy for systemic diseases. The transplantation of myoblasts into sk eletal muscle also allows gene transfer into cells of the host since m yoblasts fuse with host fibers thereby forming hybrid myofibers, The s uccess of myoblast transplantation can be determined by a variety of m easures, such as the percentage of myoblasts that fuse, the number of hybrid myofibers formed, or the level of transgene expression. Each me asure is a reflection of the fate of the transplanted cells. In order to compare different measures of transplantation efficacy, we followed the fate of transplanted myoblasts expressing the marker enzyme beta- galactosidase (beta-gal) in two different assays. Two weeks after tran splantation, the number of hybrid myofibers was determined histochemic ally, whereas transgene (beta-gal) expression was measured biochemical ly. To control for variabilities of transplantation among different an imals, we obtained both measurements from each muscle by using alterna te cryosections in the two assays, Within each individual muscle, both hybrid fiber number and beta-gal expression were maximal at the site of implantation and diminished in parallel with distance from the site . However, for determining the success of transplantation among groups of muscles, these two measures of efficacy yielded discordant results : the transplants with the highest number of hybrid fibers were not th e transplants with the greatest beta-gal activity. Such discrepancies are likely due to regional variations at the transplantation site that arise when cells are introduced into a solid tissue, These results de monstrate the importance of multiple measures of cell fate and transpl antation efficacy for studies of cell transplantation and for the appl ication of such studies to cell therapy and cell-mediated gene therapy . (C) 1995 Academic Press, Inc.