INDUCED INSECTICIDE TOLERANCE IN LARVAE OF LUCILIA-CUPRINA (WIEDEMANN) (DIPTERA, CALLIPHORIDAE) FOLLOWING DIETARY PHENOBARBITAL TREATMENT

Authors
Citation
Ac. Kotze, INDUCED INSECTICIDE TOLERANCE IN LARVAE OF LUCILIA-CUPRINA (WIEDEMANN) (DIPTERA, CALLIPHORIDAE) FOLLOWING DIETARY PHENOBARBITAL TREATMENT, Journal of the Australian Entomological Society, 34, 1995, pp. 205-209
Citations number
40
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Entomology
ISSN journal
0004-9050
Volume
34
Year of publication
1995
Part
3
Pages
205 - 209
Database
ISI
SICI code
0004-9050(1995)34:<205:IITILO>2.0.ZU;2-T
Abstract
Insecticide tolerance in the Australian sheep blowfly, Lucilia cuprina , was examined using an enzyme-induction approach. The effects of diet ary phenobarbital administration on detoxification-enzyme activities a nd insecticide tolerances were measured using first and second instars . The LC50 of butacarb to blowfly larvae increased 85-fold following p henobarbital treatment. Tolerance to diazinon and diflubenzuron also i ncreased considerably, with LC50s 12- and I-fold higher, respectively. Tolerance to deltamethrin only increased slightly (1.4-fold). The deg rees of tolerance towards butacarb and diazinon increased with increas ing concentrations of phenobarbital in the larval diet. Phenobarbital- treated larvae showed significantly enhanced cytochrome P450 levels re lative to controls. Aldrin epoxidase, P450 reductase and glutathione t ransferase activities were enhanced 168-, 1.7- and 2.2-fold, respectiv ely, in treated larvae. The induced monooxygenase and glutathione tran sferase systems appear to equip the larvae with the ability to degrade increased amounts of various types of insecticides.