DETECTION OF PROVIRAL DNA OF BOVINE LEUKEMIA-VIRUS IN CATTLE BY A COMBINATION OF IN-SITU HYBRIDIZATION AND THE POLYMERASE CHAIN-REACTION

XIE B OYAMADA T YOSHIKAWA H OYAMADA T YOSHIKAWA T

B. Xie et al., DETECTION OF PROVIRAL DNA OF BOVINE LEUKEMIA-VIRUS IN CATTLE BY A COMBINATION OF IN-SITU HYBRIDIZATION AND THE POLYMERASE CHAIN-REACTION, Journal of Comparative Pathology, 116(1), 1997, pp. 87-96

21

INGLESE

Article

Pathology,"Veterinary Sciences

Journal of Comparative Pathology → ACNP

0021-9975

116

1

1997

87 - 96

ISI

0021-9975(1997)116:1<87:DOPDOB>2.0.ZU;2-7

Bovine leukaemia virus (BLV) proviral DNA was detected in lymphocytes isolated from cattle with persistent lymphocytosis (PL) by the polymer ase chain reaction (PCR) and in-situ hybridization (ISH) with a biotin ylated PX DNA probe. Many positive cells were observed when short-term culture and a combination of ISH with PCR were used. Immunohistochemi cal examination of lymphocytes isolated from the lymph node showed tha t BLV attached mainly to surface immunoglobulins (SIg) of positive B l ymphocytes, and to a few tumour-associated antigen (TAA)-, PanT-, and CD8-positive cells and non-CD4 positive cells. Electron microscopical examination revealed colloidal gold particles within the nuclei and cy toplasm of lymphocytes. Lymphoid cells from neoplastic lymph node of e nzootic bovine leukosis (EEL) cases gave particularly strong positive signals with the ISH-PCR method. The technique of combined ISH and PCR with a biotinylated PX probe may prove useful in future studies of EB L. (C) 1997 W.B. Saunders Company Limited.