HISTOCHEMICAL-DEMONSTRATION OF DIFFERENT TYPES OF POLY-N-ACETYLLACTOSAMINE STRUCTURES IN HUMAN THYROID NEOPLASMS USING LECTINS AND ENDO-BETA-GALACTOSIDASE DIGESTION

Citation
N. Ito et al., HISTOCHEMICAL-DEMONSTRATION OF DIFFERENT TYPES OF POLY-N-ACETYLLACTOSAMINE STRUCTURES IN HUMAN THYROID NEOPLASMS USING LECTINS AND ENDO-BETA-GALACTOSIDASE DIGESTION, Histochemical Journal, 27(8), 1995, pp. 620-629
Citations number
62
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell Biology
Journal title
ISSN journal
0018-2214
Volume
27
Issue
8
Year of publication
1995
Pages
620 - 629
Database
ISI
SICI code
0018-2214(1995)27:8<620:HODTOP>2.0.ZU;2-M
Abstract
Blood-group-related antigens expressed in papillary carcinomas and oth er types of neoplasm of the human thyroid glands have been shown to be carried by poly-N-acetyllactosamines containing a linear domain susce ptible to endo-beta-galactosidase digestion. To make clear more precis ely the backbone poly-N-acetyllactosamine structures, labelled lectins specific to different types of these structures and specific to core structures with beta 1-6GlcNAc branching of N- and O-linked glycoprote ins were employed in conjunction with prior endo-beta-galactosidase di gestion on formalin-fixed, paraffin-embedded neoplasms of the human th yroid glands. Ln papillary carcinomas, Datura stramonium agglutinin (D SA) and succinyl wheat germ agglutinin (Suc-WGA) reacted most consiste ntly and frequently with papillary carcinomas from all the individuals examined. Pokeweed mitogen (PWM) likewise stained the cells of papill ary carcinomas from all the individuals examined, but in some individu als the number of lectin-reactive cells were very small. Lycoperscion esculentum aggultinin (LEA), Solanum tuberosum agglutinin (STA), Phase olus vulgaris agglutinin L (PHA-L) and Artocarpus integrifolia aggluti nin (jacalin) similarly bound to the cancer cells from most of the ind ividuals, and in these cases the number of reactive cells was usually much more restricted than was the case with DSA or PWM. Ln adenoma and other types of carcinoma, such as follicular carcinomas, these lectin s specific to poly-N-acetyllactosamine exhibited slight or no reactivi ty with the cells, whereas PHA-L and jacalin similarly bound to the ce lls of adenomas and carcinomas from most of the individuals examined. Prior digestion with endo-beta-galactosidase completely eliminated or markedly reduced the reactivity with PWM and LEA in papillary carcinom as. Reactivity with DSA, Suc-WGA, STA, PHA-L and jacalin was slightly reduced or not at all affected by Enzyme digestion. These results conf irmed that poly-N-acetyllactosamine species found in papillary carcino mas are quite different from those in other types of thyroid neoplasm, suggesting that at least three different types of poly-N-acetyllactos amine, that is, linear unbranched short and long sequences and highly branched ones are produced in these cells.