TRACTION FORCES IN LOCOMOTING CELLS

Citation
T. Oliver et al., TRACTION FORCES IN LOCOMOTING CELLS, Cell motility and the cytoskeleton, 31(3), 1995, pp. 225-240
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell Biology",Biology
Journal title
ISSN journal
0886-1544
Volume
31
Issue
3
Year of publication
1995
Pages
225 - 240
Database
ISI
SICI code
0886-1544(1995)31:3<225:TFILC>2.0.ZU;2-T
Abstract
A means of determining quantitative maps of the tractions exerted by l ocomoting cells on a substratum has been developed. This method is sim ilar to the Harris silicone substratum assay [Harris et al., 1980: Sci ence 208.177-179], but uses an improved non-wrinkling film that deform s more predictably in response to traction forces. The method also uti lizes a mathematical analysis of rubber deformation to produce the fin al map of the distribution of tractions. The resulting maps consistent ly showed that fish keratocytes exert a steady-state ''pinching'' on t he substratum, perpendicular to the cell's direction of locomotion. No significant rearward tractions were detected at or near the front edg e of the cell. Likewise, no significant forward tractions associated w ith peeling of adhesions were found at the back of the cell. A second assay uses deflection of a lightly attached glass microneedle to measu re the total force exerted by locomoting cells. Forces of approximatel y 4.5 x 10(-3) dyn were required to ''stall'' locomoting keratocytes. The implications of these findings for cell movement are discussed. (C ) 1995 Wiley-Liss, Inc.