EFFECTS OF FMLP AND LPS ON [CA2-EXUDATE POLYMORPHONUCLEAR LEUKOCYTES FOLLOWING ONSET OF INFLAMMATION(](I) OF PERITONEAL)

Citation
J. Qu et al., EFFECTS OF FMLP AND LPS ON [CA2-EXUDATE POLYMORPHONUCLEAR LEUKOCYTES FOLLOWING ONSET OF INFLAMMATION(](I) OF PERITONEAL), Journal of Periodontal Research, 30(3), 1995, pp. 153-158
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Dentistry,Oral Surgery & Medicine
ISSN journal
0022-3484
Volume
30
Issue
3
Year of publication
1995
Pages
153 - 158
Database
ISI
SICI code
0022-3484(1995)30:3<153:EOFALO>2.0.ZU;2-5
Abstract
Because a general study of activated neutrophils may have relevance to periodontal diseases and accompanying inflammation, we studied a func tion of mouse polymorphonuclear leukocytes (PMNs) that exude into the peritoneal cavity in response to inflammation caused by i.p. injection of 2% casein. The effects of E. coli-lipopolysaccharide (E-LPS) and a chemotactic factor, N-formyl-N-methionyl-N-leucyl-L-phenylalanine (FM LP), on the level of intracellular calcium ([Ca2+](i)) in these PMNs w ere examined. From analysis made with a laser cytometer (ACAS 570), th e PMNs in exudates harvested 3-9 h after the onset of inflammation wer e shown to undergo [Ca2+](i) elevation in response to 10(-6) M FMLP. T he peak concentration of[Ca2+](i) elicited by FMLP was highest in exud ate cells 6 h after casein injection. In addition, about 65% of the PM Ns in the 3-h exudate were FMLP sensitive displaying an elevated [Ca2](i), whereas more than 85% of them in 6- and 9-h exudates became FMLP sensitive. Also, the maximum level of [Ca2+](i) after FMLP stimulatio n was potentiated by pretreatment of the cells with E-LPS (0.2 mu g/ml ). The present study suggests that PMNs induced by casein injection an d appearing in mouse peritoneal exudate at different times possess sig nificantly different ability to undergo [Ca2+](i) elevation, and diffe rent susceptibility toward a chemotactic factor, FMLP.