THIAMINE PYROPHOSPHATE EFFECT AND NORMALIZED ERYTHROCYTE TRANSKETOLASE ACTIVITY RATIO IN WERNICKE-KORSAKOFF PATIENTS AND ACUTE ALCOHOLICS UNDERGOING DETOXIFICATION

Citation
Hk. Rooprai et al., THIAMINE PYROPHOSPHATE EFFECT AND NORMALIZED ERYTHROCYTE TRANSKETOLASE ACTIVITY RATIO IN WERNICKE-KORSAKOFF PATIENTS AND ACUTE ALCOHOLICS UNDERGOING DETOXIFICATION, Alcohol and alcoholism, 31(5), 1996, pp. 493-501
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Substance Abuse
Journal title
ISSN journal
0735-0414
Volume
31
Issue
5
Year of publication
1996
Pages
493 - 501
Database
ISI
SICI code
0735-0414(1996)31:5<493:TPEANE>2.0.ZU;2-V
Abstract
Thiamine deficiency may be assessed clinically by an abnormally low sp ecific erythrocyte transketolase activity and/or by an abnormally larg e activation by thiamine diphosphate in vitro (or 'TPP effect'). In th e present investigation, we report erythrocyte transketolase activatio n by TPP in acute alcoholics and Wemicke-Korsakoff patients undergoing detoxification. A new age-dependent parameter was used to improve the reliability of transketolase activity as an indicator of marginal thi amine deficiency. Thus, normalized transketolase activity ratio (NTKZ) , primary activation ratio (PAR) and further activation ratio (FAR) we re measured in 29 acute alcoholics and 12 Wemicke-Korsakoff patients u pon admission, and also on 47 control subjects. It was possible to fol low up 14 of the 29 acute alcoholics after 7 days of treatment. Twenty -one per cent of the acute alcoholics and 33% of the Wemicke-Korsakoff patients, on admission to the detoxification Unit, had NTKZ values be yond the defined critical conditions for thiamine deficiency, whereas 7% of the former and 25% of the latter had PAR values beyond these cri tical conditions. Furthermore, all three parameters were significantly different in the Wernicke-Korsakoff patients compared to the other tw o groups. The pattern of improvement of the different parameters on fo llow-up varied considerably and is difficult to explain, as only the N TKZ was statistically significant. Hence, only eight out of 14 acute a lcoholics showed improvement in NTKZ, seven showed improvement of PAR and six showed improvement of FAR after treatment. Five patients showe d improvement of both NTKZ and PAR and none of the patients showed imp rovement of all three parameters. We conclude that our findings confir m previous reports and that this modified transketolase activation tes t improves its reliability as an indicator of marginal thiamine defici ency.