PSEUDOMONAS RHODESIAE SP-NOV, A NEW SPECIES ISOLATED FROM NATURAL MINERAL WATERS

COROLER L ELOMARI M HOSTE B GILLIS M IZARD D LECLERC H

L. Coroler et al., PSEUDOMONAS RHODESIAE SP-NOV, A NEW SPECIES ISOLATED FROM NATURAL MINERAL WATERS, Systematic and applied microbiology, 19(4), 1996, pp. 600-607

31

INGLESE

Article

Microbiology,"Biothechnology & Applied Migrobiology

Systematic and applied microbiology → ACNP

0723-2020

19

4

1996

600 - 607

ISI

0723-2020(1996)19:4<600:PRSANS>2.0.ZU;2-6

Deoxyribonucleic acid relatedness studies (S1 nuclease method) showed that 7 strains of the cluster IIa previously described by Elomari et a l. (J. Appl, Bacteriol 78, 71-81, (1995)) formed a homogenous deoxyrib onucleic acid hybridization group. This group of strains forms a new s pecies: Pseudomonas rhodesiae sp. nov. A total of 67 strains represent ing known or partially characterized sprecies of the genus Pseudomonas had 7 to 48% DNA hybridization with Pseudomonas rhodesiae. This new s pecies composed of strains isolated from natural mineral waters, were Gram negative, rod shaped, and motile by means of a single polar flage llum. They were not able to accumulate poly-beta-hydroxybutyrate were capable of respiratory but not fermentative metabolism. They grew at 4 degrees C but not a 41 degrees C, produced fluorescent pigment, catal ase, cytochrome oxidase and lecithinase, and possessed arginine dihydr olase system. They did not hydrolyse gelatin and starch, and were able to use glucose, trehalose, 2-ketogluconate, inositol, L-valine and be ta-alanine. They possessed L-pyrrolidone arylamidase, L-histidyl-L-ser ine arylamidase but did not have osidase, esterase = C-12, esterase = C-14, esterase = C-16, and glycyl-L-tryptophane arylamidase. The avera ge guanine + cytosine (G + C) content of the deoxyribonucleic acid was 59 +/- 1 mol%. The type strain of P. rhodesiae has been deposited as CIP 104664(T). The clinical significance of Pseudomonas rhodesiae is u nknown.