PRECLASSIFICATION OF PSEUDOMONAS-SYRINGAE PV AVELLANAE AS PSEUDOMONAS-AVELLANAE (SPEC NOV), THE BACTERIUM CAUSING CANKER OF HAZELNUT (CORYLUS-AVELLANA L)

Citation
Jd. Janse et al., PRECLASSIFICATION OF PSEUDOMONAS-SYRINGAE PV AVELLANAE AS PSEUDOMONAS-AVELLANAE (SPEC NOV), THE BACTERIUM CAUSING CANKER OF HAZELNUT (CORYLUS-AVELLANA L), Systematic and applied microbiology, 19(4), 1996, pp. 589-595
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
0723-2020
Volume
19
Issue
4
Year of publication
1996
Pages
589 - 595
Database
ISI
SICI code
0723-2020(1996)19:4<589:POPPAA>2.0.ZU;2-8
Abstract
Fourteen Pseudomonas strains causing canker of hazelnut in Greece and Italy were compared by analysis of plasmid DNA, whole cell fatty acids , whole cell proteins, and restriction fragment patterns of 16S rRNA. Moreover, fatty acid and protein profiles, 16S rRNA sequences and perc entages of DNA similarity were compared with those of other Pseudomona s bacteria, including P. aeruginosa, P. cichorii, P. corrugata, P. vir idiflava, P. syringae pv. syringae, P. s. pv. porri, P. s. pv. phaseol icola, P. s. pv. maculicola, P. s. subsp. savastanoi and P. fluorescen s. The bacterium causing canker of hazelnut originally was named P. sy ringae pv. avellanae on the basis of biochemical tests. Strains of the hazelnut organism were very homogeneous, which was confirmed by our w ork. Only in the number of plasmids, especially in Italian strains, so me variation was observed. Fatty acid and protein profiles, as well as 16S rRNA sequence analysis and percentage of DNA-DNA hybridization wi th other Pseudomonas strains indicated that the bacterium causing cank er of hazelnut is very much apart from all pathovars of P. syringae re sted, including pv. syringae. The strains were only very distantly rel ated to P. syringae and other Pseudomonas species tested. All other Ps eudomonas species tested were closer to P. syringae than the bacterium causing canker of hazelnut. We therefore propose to reclassify P. syr ingae pv. avellanae into P. avellanae.