RIBOSOMAL-RNA-TARGETED OLIGONUCLEOTIDE PROBES FOR THE IDENTIFICATION OF GENUINE AND FORMER PSEUDOMONADS

Authors
AMANN R LUDWIG W SCHULZE R SPRING S MOORE E SCHLEIFER KH
Citation
R. Amann et al., RIBOSOMAL-RNA-TARGETED OLIGONUCLEOTIDE PROBES FOR THE IDENTIFICATION OF GENUINE AND FORMER PSEUDOMONADS, Systematic and applied microbiology, 19(4), 1996, pp. 501-509
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Systematic and applied microbiology → ACNP
ISSN journal
0723-2020
Volume
19
Issue
4
Year of publication
1996
Pages
501 - 509
Database
ISI
SICI code
0723-2020(1996)19:4<501:ROPFTI>2.0.ZU;2-P
Abstract
The specificity of previously published oligonucleotide probes war re- evaluated based on the expanded 16S and 23S rRNA databases. Moreover, new genus- and (sub)species-specific 16S rRNA-targeted oligonucleotide probes were designed in a computer-assisted strategy using the PROBE DESIGN tool of the software package ARE. Probes for Pseudomonas stutze ri and for the genera Acidovorax, Comamonas and Hydrogenophaga were ev aluated. Optimal hybridization conditions for the probes have been det ermined. Using these conditions the probes were tested for binding to 16S rRNA of reference cultures. Furthermore, fluorescently labeled der ivatives of these probes were tested for their applicability for whole cell hybridization. These rRNA-targeted probes are the basis for high -resolution, rapid, automated microbial identification.