THE DETERMINATION AND COMPARISON OF THE 16S RIBOSOMAL-RNA GENE-SEQUENCES OF SPECIES OF THE GENUS PSEUDOMONAS (SENSU-STRICTO) AND ESTIMATIONOF THE NATURAL INTRAGENERIC RELATIONSHIPS

Citation
Erb. Moore et al., THE DETERMINATION AND COMPARISON OF THE 16S RIBOSOMAL-RNA GENE-SEQUENCES OF SPECIES OF THE GENUS PSEUDOMONAS (SENSU-STRICTO) AND ESTIMATIONOF THE NATURAL INTRAGENERIC RELATIONSHIPS, Systematic and applied microbiology, 19(4), 1996, pp. 478-492
Citations number
58
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
0723-2020
Volume
19
Issue
4
Year of publication
1996
Pages
478 - 492
Database
ISI
SICI code
0723-2020(1996)19:4<478:TDACOT>2.0.ZU;2-P
Abstract
As a consolidated effort on the part of several laboratories, partial and nearly complete sequence determinations of 16S rRNA genes have bee n applied as one of several analytical methods in a polyphasic study o f the pseudomonads. Nearly-complete sequences have been determined of the PCR-amplified 16S rRNA genes of 21 species of the genus Pseudomona s (sensu stricto), including multiple attains of most species. Phyloge netic branching orders and the natural intrageneric relationships amon g the species have been infrared through sequence comparisons and clus ter analysis and have not shown any obvious recognizable correlation w ith results derived through standard phenotypic criteria commonly used to group ;he species. This paper also focuses on the ability of 16S r RNA gene sequences, particularly the hypervariable sequence regions, t o be used as nested identification markers and as target sites for the development of 16S rRNA sequence-based strategies for the identificat ion of species of the genus Pseudomonas.