PHENOTYPIC AND FUNCTIONAL-HETEROGENEITY OF THE MURINE ALVEOLAR MACROPHAGE-DERIVED CELL-LINE MH-S

Citation
K. Sankaran et Hb. Herscowitz, PHENOTYPIC AND FUNCTIONAL-HETEROGENEITY OF THE MURINE ALVEOLAR MACROPHAGE-DERIVED CELL-LINE MH-S, Journal of leukocyte biology, 57(4), 1995, pp. 562-568
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology,Hematology
ISSN journal
0741-5400
Volume
57
Issue
4
Year of publication
1995
Pages
562 - 568
Database
ISI
SICI code
0741-5400(1995)57:4<562:PAFOTM>2.0.ZU;2-B
Abstract
We have previously reported that MH-S, an established murine alveolar macrophage-derived cell line, mediated profound inhibition of in vitro antibody production, as did their freshly isolated alveolar macrophag e (AM) counterparts, In this communication we show that like freshly r ecovered AMs, the MH-S cell line also displays phenotypic and function al heterogeneity, Sorting of parental MH-S cells by flow cytometry bas ed on reactivity with anti-Mac-1 antibody yielded two subsets. Further analysis by staining with monoclonal antibodies against well-characte rized murine macrophage cell surface markers revealed that both Mac-1( +) and Mac-1(-) subsets expressed the mature murine macrophage antigen (F4/80) and class II major histocompatibility complex molecules, but with different intensity. In contrast, the two subsets stained equival ently with antibody against the Fc gamma II receptor, whereas neither subset stained with anti-CD4 antibody, Examination by light microscopy revealed pleomorphism in the Mac-1(+) population with many of the cel ls appearing spindle shaped and having elongated processes, whereas a majority of the cells in the Mac-(1)- population were spherical in sha pe. Functionally, cells from the Mac-1(+) population were less inhibit ory of in vitro antibody production and produced significantly more ni tric oxide in response to stimulation with lipopolysaccharide than wer e cells in the Mac-1(-) population. Essentially similar results were o btained using cloned Mac-1(+) and Mac-1(-) MH-S cells, The finding of heterogeneity in an established cell line that displays functions simi lar to those of freshly recovered AMs suggests that distinct subsets o f AMs may be involved in the pathogenesis of disease processes in the lung.