All diabetic patients should be screened for the early stage of diabet
ic nephropathy, because microalbuminuria has a great prognostic signif
icance, The albusure test (AT), alatex agglutination nephelometric imm
unoassay, is a rapid and low cost test for the detection of microalbum
inuria of 30mg/L or more, We compared the results of AT and of radioim
munoassay (RIA) for urinary albumin to evaluate the clinical utility o
f AT using fresh urine samples from 74 diabetic patients without persi
stent proteinuria and from 11 healthy subjects. Urinary albumin levels
measured by RIA were 6.0+/-2.3mg/L in the healthy subjects, 11.0+/-8.
7mg/L in the AT-negative group (n=61), and 38.1+/-10.2mg/L in the AT-p
ositive group (n=13). Using a cut-off value of 30mg/L by RIA, the rate
of coincidence between AT and RIA was 89.2%, although five subjects w
ere false-positive by AT, and three were false-negative, These results
show that AT may provide a useful monitor microalbuminuria, a reliabl
e early mark of diabetic nephropathy.