DISCOVERY OF EXTRACELLULAR MULTIPLE FORM OF CHLAMYDIA-TRACHOMATIS IN THE TISSUE-CULTURE

Authors
Citation
Zh. Li et al., DISCOVERY OF EXTRACELLULAR MULTIPLE FORM OF CHLAMYDIA-TRACHOMATIS IN THE TISSUE-CULTURE, Chinese medical journal, 107(9), 1994, pp. 658-663
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medicine, General & Internal
Journal title
ISSN journal
0366-6999
Volume
107
Issue
9
Year of publication
1994
Pages
658 - 663
Database
ISI
SICI code
0366-6999(1994)107:9<658:DOEMFO>2.0.ZU;2-0
Abstract
A strain of Chlamydia trachomatis was isolated from a patient with non gonococcal urethritis (NGU). Alternate passages between chick embryo a nd McCoy cell culture were examined, From the Giemsa stained coverslip s taken from the cell culture 96 hours after inoculation, we found, to our surprise, that elementary bodies (EBs) distributed over a large a rea, and several intact cells embedded in them. These pure EB particle s are round, fairly uniform in size and often appeared in pair. Accord ing to their morphology, distribution, arrangement and relationship wi th host cells, they are not the remains after cell lysis or directly r eleased from host cells. We considered that they consisted of EBs whic h continued to divide by binary fission after their release. The name ''Extracellular Multiply Form'' was designated and their formation mec hanism was proposed. This discovery gives a great challenge to primary theory, i.e. Chlamydias are obligate intracellular prokaryotic parasi tes. If we can further reveal the law of their formation, it will be o f great significance both theoretically and practically.