STIMULATORY EFFECT OF UNSATURATED FATTY-ACIDS ON THE LEVEL OF PLASMINOGEN-ACTIVATOR INHIBITOR-1 MESSENGER-RNA IN CULTURED HUMAN ENDOTHELIAL-CELLS

Citation
K. Kariko et al., STIMULATORY EFFECT OF UNSATURATED FATTY-ACIDS ON THE LEVEL OF PLASMINOGEN-ACTIVATOR INHIBITOR-1 MESSENGER-RNA IN CULTURED HUMAN ENDOTHELIAL-CELLS, FEBS letters, 361(1), 1995, pp. 118-122
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biophysics,Biology
Journal title
ISSN journal
0014-5793
Volume
361
Issue
1
Year of publication
1995
Pages
118 - 122
Database
ISI
SICI code
0014-5793(1995)361:1<118:SEOUFO>2.0.ZU;2-#
Abstract
To determine whether unsaturated fatty acids induce changes in the mRN A level of plasminogen activator inhibitor type-1 (PAI-1). Northern an alyses were performed on human umbilical vein endothelial cells (HUVEC ) and vascular smooth muscle cells that were treated with two common f atty acids. Supplementation of cultured HUVEC with docosahexanoic acid (DHA) or with dihomogamma linolenic acid (DGLA), resulted in a concen tration dependent, specific increase of the PAI-1 transcript levels, w hich was detectable within 2 h. DHA and DGLA treatment of smooth muscl e cells did not result in changes in the PAI-1 mRNA levels. Homology s earch of the upstream regulatory region of the PAI-1 gene sequences id entified a consensus nucleotide sequence for a fatty acid-responsive e lement. Our results indicate that unsaturated fatty acids selectively increase PAI-1 mRNA levels in endothelial cells, the primary source of circulating PAI-1 in vivo.