STRUCTURE OF THE TERMINAL 300 KB OF DNA FROM HUMAN-CHROMOSOME 21Q

Citation
Jt. Reston et al., STRUCTURE OF THE TERMINAL 300 KB OF DNA FROM HUMAN-CHROMOSOME 21Q, Genomics, 26(1), 1995, pp. 31-38
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Genetics & Heredity
Journal title
ISSN journal
0888-7543
Volume
26
Issue
1
Year of publication
1995
Pages
31 - 38
Database
ISI
SICI code
0888-7543(1995)26:1<31:SOTT3K>2.0.ZU;2-2
Abstract
The most distal 300 kb of human chromosome 21q was cloned and mapped u sing telomeric yeast artificial chromosomes (YACs). The region contain s low-copy subtelomeric repeats at the telomeric end, chromosome 21-sp ecific sequences more centromerically, and the S100B gene at a distanc e of 100-140 kb from the chromosome terminus. RecA-assisted restrictio n endonuclease cleavage of genomic DNA showed that the cloned fragment s correspond to telomere-terminal ge nomic DNA, and restriction enzyme mapping of the YACs shows that the smaller clone (175 kb) corresponds exactly to the telomeric end of the larger one (300 kb). PCR assays f or Sig-specific markers were used to show that COL6A1, COL6A2, and LA1 61 were all outside of the subtelomeric region spanned by the YACs and thus at least 300 kb from the 21q terminus. The molecular probes prov ide telomeric closure for existing 21q maps. (C) 1995 Academic Press, Inc.