DISTRIBUTION OF THE ORGANELLAR CA2+ TRANSPORT ATPASE SERCA2 ISOFORMS IN THE CAT BRAIN

Citation
F. Babaaissa et al., DISTRIBUTION OF THE ORGANELLAR CA2+ TRANSPORT ATPASE SERCA2 ISOFORMS IN THE CAT BRAIN, Brain research, 743(1-2), 1996, pp. 141-153
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences
Journal title
Brain research → ACNP
ISSN journal
0006-8993
Volume
743
Issue
1-2
Year of publication
1996
Pages
141 - 153
Database
ISI
SICI code
0006-8993(1996)743:1-2<141:DOTOCT>2.0.ZU;2-C
Abstract
Of the three genes encoding the Ca(2+)transport ATPases of the endopla smic reticulum, the SERCA2 gene is the major isoform expressed in the mammalian brain. The SERCA2 transcript is alternatively processed gene rating two protein isoforms: SERCA2a which is expressed in cardiac and slow-skeletal muscle, and SERCA2b, the house-keeping isoform which is ubiquitously expressed. We have studied the expression of SERCA2 in t he cat brain, and at a less refined level also in the rat brain, using antibodies specific for either SERCA2a or SERCA2b. The SERCA2a staini ng was very restricted. The SERCA2a antibody clearly labeled the cell body of the Purkinje neurons and weakly stained the giant cells of the gigantocellular reticular nuclei. In contrast, the SERCA2b isoform wa s found in most regions of the brain. It appeared to be largely confin ed to neuronal cells. Neuroglial cells were negative. The antibody sta ined the cell body. In heavily labeled cells such as the pyramidal cel ls of the hippocampus and of the cerebral cortex, it also stained the proximal portion of the dendrites. The most intense labeling was obser ved in the Purkinje neurons, which were stained all over the cell. inc luding the distal ramifications of the dendritic tree. Remarkably the SERCA2b labeling in neuronal cells of the hypothalamic area and the su bstantia nigra was very weak. The possible physiological significance of these results is discussed.