IN-VITRO HYPOXIA OF CORTICAL AND HIPPOCAMPAL CA1 NEURONS - GLUTAMATE,NITRIC-OXIDE, AND PLATELET-ACTIVATING-FACTOR PARTICIPATE IN THE MECHANISM OF SELECTIVE NEURAL DEATH IN CA1 NEURONS

Citation
T. Ohmori et al., IN-VITRO HYPOXIA OF CORTICAL AND HIPPOCAMPAL CA1 NEURONS - GLUTAMATE,NITRIC-OXIDE, AND PLATELET-ACTIVATING-FACTOR PARTICIPATE IN THE MECHANISM OF SELECTIVE NEURAL DEATH IN CA1 NEURONS, Brain research, 743(1-2), 1996, pp. 109-115
Citations number
18
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
0006-8993
Volume
743
Issue
1-2
Year of publication
1996
Pages
109 - 115
Database
ISI
SICI code
0006-8993(1996)743:1-2<109:IHOCAH>2.0.ZU;2-0
Abstract
We prepared neuron-rich cultures from cortical and hippocampal CA1 reg ions of postnatal day 1 (P1) rats. Using these cultures, we investigat ed the sensitivity of neurons to hypoxic insults. The effects of MK-80 1, cycloheximide, N-G-nitro-L-arginine (L-NNA), and anti-platelet-acti vating factor (anti-PAF) IgG on neuronal injury under hypoxic conditio ns also were examined. The percentage of astroglial cells was higher i n CA1 than cortical cultures despite use of the same culture procedure . Despite this finding, the percentage of lactate dehydrogenase (LDH) released into the medium was greater in CA1 than cortical cultures und er the conditions of 24-h hypoxia and 24-h incubation (P < 0.05). We t hen added MK-801 (500 nM), cycloheximide (3 mu M), L-NNA (100 mu M) an d anti-PAF IgG (50 mu g/ml) prior to inducing the hypoxia and measured LDH in the medium after 24-h hypoxia and 48-h incubation. Under the h ypoxic condition, MK-801, L-NNA, and anti-PAF IgG significantly protec ted the CA1 neurons from hypoxic injury compared with cortical neurons , while cycloheximide protected both cultures equally. These results s uggest that CA1 neurons are more sensitive to hypoxia than cerebral co rtical neurons, and glutamate, nitric oxide, and PAF may participate i n the mechanism of selective neural death in neurons of the CA1 region due to hypoxia.