INCREASED PROLIFERATION OF PRECURSOR CELLS IN THE ADULT-RAT BRAIN AFTER TARGETED LESIONING

Citation
De. Weinstein et al., INCREASED PROLIFERATION OF PRECURSOR CELLS IN THE ADULT-RAT BRAIN AFTER TARGETED LESIONING, Brain research, 743(1-2), 1996, pp. 11-16
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
0006-8993
Volume
743
Issue
1-2
Year of publication
1996
Pages
11 - 16
Database
ISI
SICI code
0006-8993(1996)743:1-2<11:IPOPCI>2.0.ZU;2-S
Abstract
There have been a number of reports on the proliferation of a subset o f precursor cells in the subventricular zone of the lateral ventricles in the adult mammalian brain. Here we report on studies that sought t o ascertain whether these cells could respond to a targeted lesion of the adult brain by increasing their proliferative rate. We have lesion ed the fimbria fornix, a major pathway of septal cholinergic fibers. P revious reports demonstrated that such a lesion results in the loss of neurons in both the basal forebrain and in the CA1 field of hippocamp us, without direct injury to either tissue. Ten days after making such a lesion in adult rats, the animals were given serial injections of [ H-3]thymidine and sacrificed after a final injection. The brains were processed for both immunocytochemistry and autoradiography. Our data d emonstrate a two-fold increase over the basal proliferative rate in an imals that had received such a lesion. We used a panel of antibodies t o ascertain the identity of the proliferating cells. The only clearly identifiable cells that were [H-3]thymidine-positive were astrocytes, based on GFAP staining. The remainder of the cells were of a null phen otype.