THE ANTITUMOR ACTION OF SEMINAL RIBONUCLEASE AND ITS QUATERNARY CONFORMATIONS

Citation
V. Cafaro et al., THE ANTITUMOR ACTION OF SEMINAL RIBONUCLEASE AND ITS QUATERNARY CONFORMATIONS, FEBS letters, 359(1), 1995, pp. 31-34
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
0014-5793
Volume
359
Issue
1
Year of publication
1995
Pages
31 - 34
Database
ISI
SICI code
0014-5793(1995)359:1<31:TAAOSR>2.0.ZU;2-A
Abstract
It has been previously shown that the antitumor action of bovine semin al ribonuclease (BS-RNase) is dependent on its dimeric structure. Howe ver, two distinct quaternary structures, each in equilibrium with the other, have been described for the enzyme: one in which the two subuni ts exchange their N-terminal ends, the other with no exchange. Antitum or activity assays, carried out on homogeneous quaternary forms of the enzyme, as well as on dimeric mutants of bovine pancreatic RNase A, r eveal that another structural determinant of the antitumor activity of BS-RNase is the exchange of N-terminal ends between subunits.