ACTIVATION OF PROTEIN-KINASE-C FROM GUINEA-PIG AND RAT VENTRICULAR MUSCLES BY MUSCLIDE-A1 AND ITS (2R,5S)-DIASTEREOMER

Citation
I. Kimura et al., ACTIVATION OF PROTEIN-KINASE-C FROM GUINEA-PIG AND RAT VENTRICULAR MUSCLES BY MUSCLIDE-A1 AND ITS (2R,5S)-DIASTEREOMER, PTR. Phytotherapy research, 9(1), 1995, pp. 16-20
Citations number
14
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Pharmacy
Journal title
ISSN journal
0951-418X
Volume
9
Issue
1
Year of publication
1995
Pages
16 - 20
Database
ISI
SICI code
0951-418X(1995)9:1<16:AOPFGA>2.0.ZU;2-0
Abstract
The activating effects of musclide-A1, 6-methyl-2,5-heptanediol 5-(hyd rogen sulphate) (la), and its (2R, 5S)- and (2R, SR)-diastereomers on ventricular protein kinase C from guinea-pig and rat were compared wit h other natural compounds derived from musk and non-natural related co mpounds, The (2R, 5S)-diastereomer was most potent among musk-derived compounds and its related non-natural compounds. The activation by la (20 mu M) was produced in the presence of low [Ca2+](0) and phosphatid ylserine like 1,2-dioctanoylglycerol (2 mu M). The activation pattern for peaks eluted by a hydroxyapatite column demonstrated that la activ ates ventricular-specific protein kinase C peak II, suggesting one mec hanism for the beta-adrenergic cardiotonic potentiation by 1a.