EVIDENCE FOR POSTTRANSCRIPTIONAL REGULATION OF CYCLIN B1 MESSENGER-RNA IN THE CELL-CYCLE AND FOLLOWING IRRADIATION IN HELA-CELLS

Citation
A. Maity et al., EVIDENCE FOR POSTTRANSCRIPTIONAL REGULATION OF CYCLIN B1 MESSENGER-RNA IN THE CELL-CYCLE AND FOLLOWING IRRADIATION IN HELA-CELLS, EMBO journal, 14(3), 1995, pp. 603-609
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology
Journal title
ISSN journal
0261-4189
Volume
14
Issue
3
Year of publication
1995
Pages
603 - 609
Database
ISI
SICI code
0261-4189(1995)14:3<603:EFPROC>2.0.ZU;2-4
Abstract
Cyclin B1 mRNA expression varies markedly through the cell cycle with its peak in G(2)/M and lowest level in G(1). Cyclin B1 mRNA levels are also transiently reduced in HeLa cells after gamma-irradiation, coinc ident with the radiation-induced G(2) block. In order to understand th e mechanisms underlying these variations, we have measured cyclin B1 m RNA stability in HeLa cells during different phases of the cell cycle. The half-life of the mRNA measured after actinomycin D administration is 1.1-1.8 h in both early and late G(1), 8 h in S and 13 h in G(2)/M . We therefore conclude that altered RNA stability is important in mod ulating cyclin B1 mRNA levels through the HeLa cell cycle. Furthermore , 3 h after irradiation of HeLa cells in S phase with 10 Gy, the half- life of cyclin B1 mRNA is reduced to 5 h; it is further reduced to 2-3 h at 14 h after irradiation. Thus, decreased stability contributes to the reduction in cyclin B1 mRNA following irradiation.