STUDIES ON THE QUANTITATIVE AUTORADIOGRAPHY .3. QUANTITATIVE COMPARISON OF A NOVEL TISSUE MOLD MEASUREMENT TECHNIQUE PASTE MOLD METHOD, TO THE SEMIQUANTITATIVE WHOLE-BODY AUTORADIOGRAPHY (WBA), USING THE SAME ANIMALS

Citation
N. Motoji et al., STUDIES ON THE QUANTITATIVE AUTORADIOGRAPHY .3. QUANTITATIVE COMPARISON OF A NOVEL TISSUE MOLD MEASUREMENT TECHNIQUE PASTE MOLD METHOD, TO THE SEMIQUANTITATIVE WHOLE-BODY AUTORADIOGRAPHY (WBA), USING THE SAME ANIMALS, Biological & pharmaceutical bulletin, 18(1), 1995, pp. 100-107
Citations number
9
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Pharmacy
ISSN journal
0918-6158
Volume
18
Issue
1
Year of publication
1995
Pages
100 - 107
Database
ISI
SICI code
0918-6158(1995)18:1<100:SOTQA.>2.0.ZU;2-P
Abstract
A novel preparation technique, so called ''Paste Mold,'' was devised f or organ and tissue distribution studies. This is the most powerful by joining with autoradioluminography (ARLG), which was established and validated recently in the working group of Forum '93 of Japanese Socie ty for study of xenobiotics. A small piece (10-50 mg) of each organ or tissue was available for measuring its radioactive concentration and it was sampled from the remains of frozen carcass used for macroautora diography (MARG). The solubilization of the frozen pieces was performe d with mixing a suitable volume of gelatine and strong alkaline soluti on prior to mild heating kept at 40 degrees C for a few hours. After t hat, the tissue paste was molded in template pattern to form the small plates. The molded plates were contacted with Imaging plate (IP) for recording their radioactive concentration. The recorded IP was process ed by BAS2000. The molded plate was formed in thickness of 200 mu m, s o called infinit thickness against soft beta rays, and therefore the r esulting relative intensities, represented by (PSL-BG)/S values, indic ated practically responsible ratio of the radioactive concentration in organs and tissues, without any calibulation for beta-self absorption coefficiency. On the other hand, the left half body of the frozen car cass was used for making whole body autoradiography (WBA) before the P aste-Mold preparation. Comparison was performed for difference in (PSL -BG)/S values of organs and tissues between frozen and dried sections. A good concordance in relative intensities, (PSL-BG)/S by the Paste-M old preparation was given with those by the frozen sections rather tha n dried sections. The combination of the Paste-Mold preparation prior to ARLG and WBA method must be the most powerful to give a quantitativ e and reliable data in the tissue distribution study.