ALCOHOL-DEHYDROGENASE FROM HUMAN STOMACH - VARIABILITY IN NORMAL MUCOSA AND EFFECT OF AGE, GENDER, ADH(3), PHENOTYPE AND GASTRIC REGION

Citation
A. Moreno et al., ALCOHOL-DEHYDROGENASE FROM HUMAN STOMACH - VARIABILITY IN NORMAL MUCOSA AND EFFECT OF AGE, GENDER, ADH(3), PHENOTYPE AND GASTRIC REGION, Alcohol and alcoholism, 29(6), 1994, pp. 663
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Substance Abuse
Journal title
ISSN journal
0735-0414
Volume
29
Issue
6
Year of publication
1994
Database
ISI
SICI code
0735-0414(1994)29:6<663:AFHS-V>2.0.ZU;2-3
Abstract
Alcohol dehydrogenase (ADH) has been analysed in 36 endoscopic biopsie s of normal gastric body and/or antrum mucosa, from 31 individuals wit h an age between 17 and 79 years. Oesophageal, duodenal and oral mucos a specimens have been also examined. Stomach mucosa contains three iso zyme types: the gamma gamma-ADH forms (class I), sigma sigma-ADH (clas s IV) and chi chi-ADH (class III). gamma gamma-ADH was present in all gastric samples, while sigma sigma-ADH was detected in all body specim ens (n = 15) but only in eight of 20 antrum biopsies. The presence of high sigma sigma-ADH activity in oral and oesophageal mucosa confirms the distribution of class IV in the upper gastrointestinal tract where it may serve as a first metabolic barrier against ingested alcohols a nd aldehydes. Considering all gastric specimens, ADH activity was 5.78 +/- 2.61 mU/mg of protein with 100 mM ethanol, pH 10.0. ADH activity was higher in men than women and in gastric body than in antrum, altho ugh differences did not reach statistical significance. However, activ ity was significantly higher in subjects below 50 years than those old er than 50 years. Furthermore, an inverse correlation was found betwee n gastric ADH activity and age (r = -0.40, P < 0.02). In old subjects ADH activity was significantly higher in gastric body (5.87 +/- 1.22 m U/mg) than in antrum (4.14 +/- 1.78 mU/mg) (P = 0.03). Differences in activity between samples from each ADH(3) phenotype were statistically not significant but corresponded to those expected from the kinetic c onstants of the respective gamma gamma-ADH isozymes, which suggests th at ADH(3) polymorphism affects activity. We conclude that age has a ma jor effect on gastric ADH, while stomach region and ADH(3) phenotype a lso influence its activity.