NUCLEAR MORPHOMETRY IN AUTOMATIC BIOPSY AND RADICAL PROSTATECTOMY SPECIMENS OF PROSTATIC-CARCINOMA - A COMPARISON

Citation
Jl. Mohler et al., NUCLEAR MORPHOMETRY IN AUTOMATIC BIOPSY AND RADICAL PROSTATECTOMY SPECIMENS OF PROSTATIC-CARCINOMA - A COMPARISON, Analytical and quantitative cytology and histology, 16(6), 1994, pp. 415-420
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell Biology
ISSN journal
0884-6812
Volume
16
Issue
6
Year of publication
1994
Pages
415 - 420
Database
ISI
SICI code
0884-6812(1994)16:6<415:NMIABA>2.0.ZU;2-W
Abstract
Nuclear shape analysis performed upon prostatectomy specimens of prost atic carcinoma distinguished individual patients with good or poor pro gnoses. In order to be useful for prognosis assessment preoperatively, nuclear morphometry must be measured on needle biopsy specimens. We c ompared nuclear morphomtery on automatic biopsy and radical prostatect omy specimens in 20 patients with prostatic carcinoma. Nuclear size wa s smaller (paired Student t test, P<.0001) in biopsy specimens (perime ter 17.0+/-SD 4.9 mu m, area 29.9+/-6.6 mu m(2)) than in prostatectomy specimens (perimeter 24.6+/-4.4 mu m, area 48.2+/-8.7 mu m(2)). Nucle ar shape was move abnormal in automatic biopsy specimens (nuclear roun dness factor 82.0 +/- 18.8, ellipticity 90.5 +/- 27.7) than in surgica l specimens (nuclear roundness factor 43.5 +/- 8.8, ellipticity 54.0 /- 14.7) (P<.0001). Study of specimens obtained by automatic biopsy pr eoperatively and automatic biopsy of the prostatectomy specimens at va rious steps of processing revealed that nuclear swelling and rounding occurred after 2-24 hours of formalin fixation. Automatic prostate bio psies may more accurately reflect true nuclear morphometry and should be studied for preoperative prognosis prediction in patients with clin ically localized prostatic carcinoma.