STEROID-HORMONE MODULATION OF VITAMIN-D-RECEPTOR LEVELS IN HUMAN MG-63 OSTEOSARCOMA CELLS

Citation
A. Mahonen et Ph. Maenpaa, STEROID-HORMONE MODULATION OF VITAMIN-D-RECEPTOR LEVELS IN HUMAN MG-63 OSTEOSARCOMA CELLS, Biochemical and biophysical research communications, 205(2), 1994, pp. 1179-1186
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Biophysics
ISSN journal
0006-291X
Volume
205
Issue
2
Year of publication
1994
Pages
1179 - 1186
Database
ISI
SICI code
0006-291X(1994)205:2<1179:SMOVLI>2.0.ZU;2-3
Abstract
The effects of steroid and thyroid hormones are mediated by intracellu lar hormone receptors. An important mechanism modulating target tissue responsiveness to hormones is homologous and heterologous regulation of the receptors. We have characterized the expression of steroid horm one receptors in human MG-63 osteosarcoma cells. The MG-63 cells expre ss receptor mRNAs for glucocorticoids, estrogen, retinoic acid, and 1, 25(OH)(2)D-3. We found that only the vitamin D receptor (VDR) mRNA con centration was influenced by the hormones. The stability of the VDR me ssage was identical in control, dexamethasone- and estradiol-treated c ells. On the other hand, both 1,25(OH)(2)D-3 and retinoic acid separat ely stabilized the VDR mRNA levels increasing the apparent half-life b y 11 h and 6 h, respectively. The VDR protein levers, however, as meas ured by immunoprecipitation, increased only after the 1,25(OH)(2)D-3 t reatment. (C) 1994 Academic Press, Inc.