THE COMBINED USE OF WHOLE-CELL PROTEIN EXTRACTS FOR THE IDENTIFICATION (SDS-PAGE) AND ENZYME-ACTIVITY SCREENING OF LACTIC-ACID BACTERIA ISOLATED FROM TRADITIONAL GREEK DAIRY-PRODUCTS

Citation
E. Tsakalidou et al., THE COMBINED USE OF WHOLE-CELL PROTEIN EXTRACTS FOR THE IDENTIFICATION (SDS-PAGE) AND ENZYME-ACTIVITY SCREENING OF LACTIC-ACID BACTERIA ISOLATED FROM TRADITIONAL GREEK DAIRY-PRODUCTS, Systematic and applied microbiology, 17(3), 1994, pp. 444-458
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
0723-2020
Volume
17
Issue
3
Year of publication
1994
Pages
444 - 458
Database
ISI
SICI code
0723-2020(1994)17:3<444:TCUOWP>2.0.ZU;2-Z
Abstract
A total of 148 strains of lactic acid bacteria from the ACA-DC Collect ion were examined in this study. They were all isolated from tradition al Greek dairy products such as yoghurt and cheese and were assigned t o the genera Enterococcus, Lactobacillus, Lactococcus, Leuconostoc and Streptococcus. By use of traditional phenotypic methods 65 strains we re identified to the species level; the remaining 83 strains were part ially characterised (genus level). The SDS-PAGE technique of whole-cel l proteins was evaluated as an advanced tool for the identification of lactic acid bacteria. Therefore protein fingerprints were registered for all strains and compared to a large number of lactic acid bacteria reference strains stored in database format. In most cases the SDS-PA GE analysis confirmed the phenotypic identification results. With only a few exceptions, all partially characterised strains were identified to (sub)species level. In order to quickly reveal characteristics whi ch could be important for their use as starter cultures in dairy ferme ntations, it is shown that the bacterial cell-free protein extracts co uld simultaneously be screened for peptidase and esterase activities u sing synthetic substrates. Obvious differences among the strains, the groups of strains as well as on the genus level were observed.