TAXON SPECIFIC HYBRIDIZATION PROBES FOR FIBER-DIGESTING BACTERIA SUGGEST NOVEL GUT-ASSOCIATED FIBROBACTER

Citation
Cz. Lin et al., TAXON SPECIFIC HYBRIDIZATION PROBES FOR FIBER-DIGESTING BACTERIA SUGGEST NOVEL GUT-ASSOCIATED FIBROBACTER, Systematic and applied microbiology, 17(3), 1994, pp. 418-424
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
0723-2020
Volume
17
Issue
3
Year of publication
1994
Pages
418 - 424
Database
ISI
SICI code
0723-2020(1994)17:3<418:TSHPFF>2.0.ZU;2-C
Abstract
Six oligonucleotide probes complementary to the 16S rRNAs of members o f the genus Fibrobacter were designed for identification at the levels of genus, species and subspecies. The temperature of dissociation (T- d) of probe-target hybrid was determined for each probe and used to co ntrol the stringency of hybridization. Specificity was demonstrated by comparing hybridization to rRNA from target and nontarget species imm obilized on nylon membranes. Hybridization to nucleic acid isolated fr om cable and goat gut contents suggested greater genetic diversity amo ng this assemblage of fiber-digesting bacteria than now represented in pure culture.