RANDOM AMPLIFIED POLYMORPHIC DNA OF A GROUP OF HALOPHILIC ARCHAEAL ISOLATES

Citation
Aj. Martinezmurcia et F. Rodriguezvalera, RANDOM AMPLIFIED POLYMORPHIC DNA OF A GROUP OF HALOPHILIC ARCHAEAL ISOLATES, Systematic and applied microbiology, 17(3), 1994, pp. 395-401
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
0723-2020
Volume
17
Issue
3
Year of publication
1994
Pages
395 - 401
Database
ISI
SICI code
0723-2020(1994)17:3<395:RAPDOA>2.0.ZU;2-8
Abstract
Random amplified polymorphic DNA (RAPD) can be used to characterize cl osely related bacterial strains. In the present study, we have applied RAPD to some strains of the halophilic archaeon Haloferax mediterrane i in an attempt to evaluate the newly developed method and to compare the results with those obtained from pulsed-field gel electrophoresis (PFGE) studies. The RAPD analysis correlated very well with analyses o btained from PFGE. Our results indicate that RAPD is a useful techniqu e to type the total genome with a resolution similar to that of PFGE, but with the advantage of rapidity and simplicity.