RAPID DISTINCTION OF BREVIBACTERIUM SPECIES BY RESTRICTION ANALYSIS OF RDNA GENERATED BY POLYMERASE CHAIN-REACTION

Citation
A. Carlotti et G. Funke, RAPID DISTINCTION OF BREVIBACTERIUM SPECIES BY RESTRICTION ANALYSIS OF RDNA GENERATED BY POLYMERASE CHAIN-REACTION, Systematic and applied microbiology, 17(3), 1994, pp. 380-386
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
0723-2020
Volume
17
Issue
3
Year of publication
1994
Pages
380 - 386
Database
ISI
SICI code
0723-2020(1994)17:3<380:RDOBSB>2.0.ZU;2-7
Abstract
Twelve reference strains, including the type strains, of species eithe r belonging or related to the genus Brevibacterium, and nineteen well- characterized clinical isolates assigned to this genus were studied. P olymerase chain reaction was used for partial amplification of the gen e coding for their 16S rRNA (rDNA genes) which were digested with nine different restriction endonucleases. The amplified rDNAs were approxi mately 1300 bp long. The type strains of B. casei and B. epidermidis e xhibited different BstNI rDNA restriction patterns. Furthermore, these patterns differed from those observed for B. linens and B. iodinum. O f the clinical isolates fifteen had the same BstNI restriction pattern as the B. casei type strain, two the same as the B. epidermidis type strain, one the same as B. linens, and one strain had a particular pat tern. Results obtained by rDNA restriction analysis were confirmed by DNA-DNA hybridization studies, which revealed that representatives of the fifteen isolates resembling B. casei shared more than 75% homology with B. casei CIP102111(T). Combined with a rapid DNA extraction proc edure, PCR-amplified-rDNA restriction fragment analysis allowed the di stinction of the different Brevibacterium species in less than seven h ours. The results suggested that most brevibacteria from clinical samp les belongs to B. casei rather than B. epidermidis, as commonly though t. This approach may also be of value for differentiation within other genera of Gram-positive bacteria.