COMPARISON OF RIBOTYPING ANALYSIS AND NUMERICAL TAXONOMY STUDIES OF PSEUDOMONAS-PUTIDA BIOVAR A

Citation
M. Elomari et al., COMPARISON OF RIBOTYPING ANALYSIS AND NUMERICAL TAXONOMY STUDIES OF PSEUDOMONAS-PUTIDA BIOVAR A, Systematic and applied microbiology, 17(3), 1994, pp. 361-369
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
0723-2020
Volume
17
Issue
3
Year of publication
1994
Pages
361 - 369
Database
ISI
SICI code
0723-2020(1994)17:3<361:CORAAN>2.0.ZU;2-D
Abstract
Numerical phenotypic analysis, using the unweighted pair group average linkage method and Dice similarity coefficient, was performed on 39 s trains of clinical origin belonging or related to Pseudomonas putida b iovar A and 19 collection strains belonging to the fluorescent members of Pseudomonas rRNA group I (Palleroni, 1984). Five main phenotypic c lusters (I to V) were obtained. Phenotypic cluster II (37 strains) was identified as Pseudomonas putida biovar A and it could be subdivided into 4 phenotypic subclusters (IIa to IId). The 37 strains of phenotyp ic cluster II were then characterized by ribosomal ribonucleic acid (r RNA) gene restriction patterns, after digestion of whole-cell DNA by P vu II. A pattern of hybridized fragments was obtained from each DNA st udied. Numerical analysis was then performed and seven ribotypic clust ers (A to G) were described in phenotypic cluster II. We noted the tot al correlation between phenotypic subclusters IIa and IId and ribotypi c clusters A and B respectively. Phenotypic subclusters IIb and IIc co uld be divided into 3 (E, F and G) and 2 (C and D) ribotypic clusters respectively.