COMPARISON OF RIBOTYPING ANALYSIS AND NUMERICAL TAXONOMY STUDIES OF PSEUDOMONAS-PUTIDA BIOVAR A

ELOMARI M IZARD D VINCENT P COROLER L LECLERC H

M. Elomari et al., COMPARISON OF RIBOTYPING ANALYSIS AND NUMERICAL TAXONOMY STUDIES OF PSEUDOMONAS-PUTIDA BIOVAR A, Systematic and applied microbiology, 17(3), 1994, pp. 361-369

16

INGLESE

Article

Microbiology,"Biothechnology & Applied Migrobiology

Systematic and applied microbiology → ACNP

0723-2020

17

3

1994

361 - 369

ISI

0723-2020(1994)17:3<361:CORAAN>2.0.ZU;2-D

Numerical phenotypic analysis, using the unweighted pair group average linkage method and Dice similarity coefficient, was performed on 39 s trains of clinical origin belonging or related to Pseudomonas putida b iovar A and 19 collection strains belonging to the fluorescent members of Pseudomonas rRNA group I (Palleroni, 1984). Five main phenotypic c lusters (I to V) were obtained. Phenotypic cluster II (37 strains) was identified as Pseudomonas putida biovar A and it could be subdivided into 4 phenotypic subclusters (IIa to IId). The 37 strains of phenotyp ic cluster II were then characterized by ribosomal ribonucleic acid (r RNA) gene restriction patterns, after digestion of whole-cell DNA by P vu II. A pattern of hybridized fragments was obtained from each DNA st udied. Numerical analysis was then performed and seven ribotypic clust ers (A to G) were described in phenotypic cluster II. We noted the tot al correlation between phenotypic subclusters IIa and IId and ribotypi c clusters A and B respectively. Phenotypic subclusters IIb and IIc co uld be divided into 3 (E, F and G) and 2 (C and D) ribotypic clusters respectively.