CLONING AND SEQUENCING OF A HEMAGGLUTININ COMPONENT OF THE BOTULINUM NEUROTOXIN COMPLEX ENCODED BY CLOSTRIDIUM-BOTULINUM TYPE-A AND TYPE-B

Citation
Ak. East et al., CLONING AND SEQUENCING OF A HEMAGGLUTININ COMPONENT OF THE BOTULINUM NEUROTOXIN COMPLEX ENCODED BY CLOSTRIDIUM-BOTULINUM TYPE-A AND TYPE-B, Systematic and applied microbiology, 17(3), 1994, pp. 306-312
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
0723-2020
Volume
17
Issue
3
Year of publication
1994
Pages
306 - 312
Database
ISI
SICI code
0723-2020(1994)17:3<306:CASOAH>2.0.ZU;2-6
Abstract
Degenerate oligonucleotide primers designed to the N-terminal amino ac id sequence of proteins purified from the botulinum neurotoxin complex were used to amplify DNA fragments using PCR from genomic DNA of Clos tridium botulinum types A: NCTC 7272, and B: NCTC 7273 (proteolytic st rain) and Eklund 17B (non-proteolytic strain). A fragment of similar t o 1.9 kb was amplified using template DNA from each strain, which was subsequently cloned in E. coli and the sequences determined. Two open reading frames (orfs) were discovered: one corresponding to a protein similar to 33.7 kD which shows between 36 and 41% identity with the ma jor hemagglutinin component (HA-33) of the botulinum progenitor comple x encoded by C. botulinum type C. The second orf, encoding a protein o f similar to 21.7 kD, P-21, of 178 amino acids (179 in strain NCTC 727 3) has similar to 93% identity between the type A and two type B strai ns, but is absent from type C.