NEOGENIN, AN AVIAN CELL-SURFACE PROTEIN EXPRESSED DURING TERMINAL NEURONAL DIFFERENTIATION, IS CLOSELY-RELATED TO THE HUMAN TUMOR-SUPPRESSOR MOLECULE DELETED IN COLORECTAL-CANCER

Citation
J. Vielmetter et al., NEOGENIN, AN AVIAN CELL-SURFACE PROTEIN EXPRESSED DURING TERMINAL NEURONAL DIFFERENTIATION, IS CLOSELY-RELATED TO THE HUMAN TUMOR-SUPPRESSOR MOLECULE DELETED IN COLORECTAL-CANCER, The Journal of cell biology, 127(6), 1994, pp. 2009-2020
Citations number
68
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell Biology
Journal title
ISSN journal
0021-9525
Volume
127
Issue
6
Year of publication
1994
Part
2
Pages
2009 - 2020
Database
ISI
SICI code
0021-9525(1994)127:6<2009:NAACPE>2.0.ZU;2-Z
Abstract
Using a monoclonal antibody, we have identified and characterized a pr eviously unknown cell surface protein in chicken that we call neogenin and have determined its primary sequence. The deduced amino acid sequ ence and structure of neogenin characterize it as a member of the immu noglobulin (Ig) superfamily. Based on amino acid sequence similarities , neogenin is closely related to the human tumor suppresser molecule D CC (deleted in colorectal cancer). Neogenin and DCC define a subgroup of Ig superfamily proteins structurally distinct from other Ig molecul es such as N-CAM, Ng-CAM, and Bravo/Nr-CAM. As revealed by antibody st aining of tissue sections and Western blots, neogenin expression corre lates with the onset of neuronal differentiation. Neogenin is also fou nd on cells in the lower gastrointestinal tract of embryonic chickens. DCC has been observed in human neural tissues and has been shown to b e essential for terminal differentiation of specific cell types in the adult human colon. These parallels suggest that neogenin, like DCC, i s functionally involved in the transition from cell proliferation to t erminal differentiation of specific cell types. Since neogenin is expr essed on growing neurites and downregulated at termination of neurite growth, it may also play an important role in many of the complex func tional aspects of neurite extension and intercellular signaling.