TRACTION FORCES GENERATED BY LOCOMOTING KERATOCYTES

Citation
J. Lee et al., TRACTION FORCES GENERATED BY LOCOMOTING KERATOCYTES, The Journal of cell biology, 127(6), 1994, pp. 1957-1964
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell Biology
Journal title
ISSN journal
0021-9525
Volume
127
Issue
6
Year of publication
1994
Part
2
Pages
1957 - 1964
Database
ISI
SICI code
0021-9525(1994)127:6<1957:TFGBLK>2.0.ZU;2-F
Abstract
Traction forces produced by moving fibroblasts have been observed as d istortions in flexible substrata including wrinkling of thin, silicone rubber films. Traction forces generated by fibroblast lamellae were t hought to represent the forces required to move the cell forwards. How ever, traction forces could not be detected with faster moving cell ty pes such as leukocytes and growth cones (Harris, A. K., D. Stopak, and P. Wild. 1981. Nature (Lend.). 290:249-251). We have developed a new assay in which traction forces produced by rapidly locomoting fish ker atocytes can be detected by the two-dimensional displacements of small beads embedded in the plane of an elastic substratum. Traction forces were not detected at the rapidly extending front edge of the cell. In stead the largest traction forces were exerted perpendicular to the le ft and right cell margins. The maximum traction forces exerted by kera tocytes were estimated to be similar to 2 X 10(-8) N. The pattern of t raction forces can be related to the locomotion of a single keratocyte in terms of lamellar contractility and area of close cell-substratum contact.