RESTRICTED MOVEMENT OF LIPID AND AQUEOUS DYES THROUGH PORES FORMED BYINFLUENZA HEMAGGLUTININ DURING CELL-FUSION

Citation
J. Zimmerberg et al., RESTRICTED MOVEMENT OF LIPID AND AQUEOUS DYES THROUGH PORES FORMED BYINFLUENZA HEMAGGLUTININ DURING CELL-FUSION, The Journal of cell biology, 127(6), 1994, pp. 1885-1894
Citations number
44
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell Biology
Journal title
ISSN journal
0021-9525
Volume
127
Issue
6
Year of publication
1994
Part
2
Pages
1885 - 1894
Database
ISI
SICI code
0021-9525(1994)127:6<1885:RMOLAA>2.0.ZU;2-U
Abstract
The fusion of cells by influenza hemagglutinin (HA) is the best charac terized example of protein-mediated membrane fusion. In simultaneous m easurements of pairs of assays for fusion, we determined the order of detectable events during fusion. Fusion pore formation in HA-triggered cell-cell fusion was first detected by changes in cell membrane capac itance, next by a flux of fluorescent lipid, and finally by flux of aq ueous fluorescent dye. Fusion pore conductance increased by small step s. A retardation of lipid and aqueous dyes occurred during fusion pore fluctuations. The flux of aqueous dye depended on the size of the mol ecule. The lack of movement of aqueous dyes while total fusion pore co nductance increased suggests that initial HA-triggered fusion events a re characterized by the opening of multiple small pores: the formation of a ''sieve''.