NOVEL COMPOUND TETRANUCLEOTIDE, DINUCLEOTIDE MICROSATELLITE POLYMORPHISM IN THE TUMOR NECROSIS FACTOR-LYMPHOTOXIN LOCUS

Citation
Sj. Greenberg et al., NOVEL COMPOUND TETRANUCLEOTIDE, DINUCLEOTIDE MICROSATELLITE POLYMORPHISM IN THE TUMOR NECROSIS FACTOR-LYMPHOTOXIN LOCUS, Clinical and diagnostic laboratory immunology, 4(1), 1997, pp. 79-84
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology,"Infectious Diseases","Medical Laboratory Technology",Microbiology
ISSN journal
1071-412X
Volume
4
Issue
1
Year of publication
1997
Pages
79 - 84
Database
ISI
SICI code
1071-412X(1997)4:1<79:NCTDMP>2.0.ZU;2-4
Abstract
A polymorphic (TGCG)(n) tetranucleotide repeat was discovered juxtapos ed to the (GT)(n) dinucleotide repeat that comprises the tumor necrosi s factor a microsatellite (TNFa) located telomeric to the tumor necros is factor/lymphotoxin gene cluster. The degree of complexity of this c ompound tetra-, dinucleotide microsatellite consists of 16 potential a lleles of combined length ranging from 24 to 54 bp. The pattern of fre quencies of individual alleles belonging to the compound TNFa microsat ellite was established from 52 healthy volunteers and was found to be highly heterogeneous. The data diverges significantly from previously published statistics that recognized only a simple variable dinucleoti de tandem repeat. The newly recognized compound tetra-, dinucleotide T NFa microsatellite polymorphism establishes a more accurate genetic ba sis to explore potential linkage with disease susceptibility genes loc ated within this region of the class III major histocompatibility comp lex. In addition, variable tumor necrosis factor and lymphotoxin produ ction may reflect the more complex polymorphic nature of this microsat ellite region. Finally, compound microsatellites probably exist elsewh ere, throughout the human genome. Recognition of their presence may ha ve a considerable impact on the validity of past and future microsatel lite-based genetic analyses.