REGULATION OF STEROL CARRIER PROTEIN-2 (SCP2) GENE-EXPRESSION IN RAT PERITONEAL-MACROPHAGES DURING FOAM CELL-FORMATION - A KEY ROLE FOR FREE-CHOLESTEROL CONTENT

Citation
A. Hirai et al., REGULATION OF STEROL CARRIER PROTEIN-2 (SCP2) GENE-EXPRESSION IN RAT PERITONEAL-MACROPHAGES DURING FOAM CELL-FORMATION - A KEY ROLE FOR FREE-CHOLESTEROL CONTENT, The Journal of clinical investigation, 94(6), 1994, pp. 2215-2223
Citations number
59
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medicine, Research & Experimental
ISSN journal
0021-9738
Volume
94
Issue
6
Year of publication
1994
Pages
2215 - 2223
Database
ISI
SICI code
0021-9738(1994)94:6<2215:ROSCP(>2.0.ZU;2-C
Abstract
Sterol carrier protein 2 (SCP2) has been shown to be involved in intra cellular transport and metabolism of cholesterol. However, there have been no reports concerning SCP2 in macrophages, the major source of at heromatous foam cells, We investigated whether SCP2 is present in rat peritoneal macrophages and determined the changes of SCP2 and its mRNA levels in macrophages during foam cell formation induced by acetylate d LDL (AcLDL). Immunoblot analysis and Northern blot analysis demonstr ated that both SCP2 and its mRNA are expressed in rat peritoneal macro phages. Incubations with AcLDL caused a dose- and time-dependent incre ase of cellular esterified cholesterol, SCP2 and its mRNA in rat perit oneal macrophages. The inhibitor of acyl-CoA:cholesterol acyltransfera se further enhanced AcLDL-induced increase of SCP2 protein and its mRN A. Incubations with 25-hydroxy cholesterol also caused a dose-dependen t stimulation of SCP2 gene expression in macrophages, while incubation with maleylated BSA had no effect. These results suggest that the inc rement of cellular-free cholesterol is responsible for enhanced SCP2 g ene expression in macrophages. The enhancement of SCP2 gene expression by AcLDL suggests that SCP2 may play an important role during foam ce ll formation induced by AcLDL which may be most important step for the atherosclerosis.