IN-VITRO SELECTION OF ENDOSULFAN-TOLERANT STRAINS OF BRASSICA-CAMPESTRIS (CV BROWN-SARSON)

Citation
Kr. Arya et al., IN-VITRO SELECTION OF ENDOSULFAN-TOLERANT STRAINS OF BRASSICA-CAMPESTRIS (CV BROWN-SARSON), Plant cell reports, 14(2-3), 1994, pp. 137-140
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences
Journal title
ISSN journal
0721-7714
Volume
14
Issue
2-3
Year of publication
1994
Pages
137 - 140
Database
ISI
SICI code
0721-7714(1994)14:2-3<137:ISOESO>2.0.ZU;2-V
Abstract
Endosulfan tolerant lines of mustard (Brassica campestris cv. Brown Sa rson) have been developed through tissue culture methods. Cotyledonary explants excised from eight day old in vitro grown seedlings were use d for inducing callus. Fast growing friable callus was then transferre d to MS medium containing (0.1-2.0 ugl(-1)) endosulfan for selection. Five alternating exposures with and without endosulfan containing medi um yielded an endosulfan tolerant cell line (ETL). The plants regenera ted from ETL were found to tolerate three fold higher concentrations o f endosulfan. Callus induced from randomly selected endosulfan toleran t regenerated plants were also tolerant to 3.0 ugl(-1) endosulfan, the reby, suggesting that tolerance has been acquired at the gene level. B iochemical investigation revealed higher levels of total free sugar, f ree amino acids, protein and activity of peroxidase in the tolerant ce ll line.