Kr. Arya et al., IN-VITRO SELECTION OF ENDOSULFAN-TOLERANT STRAINS OF BRASSICA-CAMPESTRIS (CV BROWN-SARSON), Plant cell reports, 14(2-3), 1994, pp. 137-140
Endosulfan tolerant lines of mustard (Brassica campestris cv. Brown Sa
rson) have been developed through tissue culture methods. Cotyledonary
explants excised from eight day old in vitro grown seedlings were use
d for inducing callus. Fast growing friable callus was then transferre
d to MS medium containing (0.1-2.0 ugl(-1)) endosulfan for selection.
Five alternating exposures with and without endosulfan containing medi
um yielded an endosulfan tolerant cell line (ETL). The plants regenera
ted from ETL were found to tolerate three fold higher concentrations o
f endosulfan. Callus induced from randomly selected endosulfan toleran
t regenerated plants were also tolerant to 3.0 ugl(-1) endosulfan, the
reby, suggesting that tolerance has been acquired at the gene level. B
iochemical investigation revealed higher levels of total free sugar, f
ree amino acids, protein and activity of peroxidase in the tolerant ce
ll line.