THE ROLE OF SECRETORY GRANULES IN THE RADIOSENSITIVITY OF RAT SALIVARY-GLAND ACINI - A MORPHOLOGICAL-STUDY

Citation
B. Peter et al., THE ROLE OF SECRETORY GRANULES IN THE RADIOSENSITIVITY OF RAT SALIVARY-GLAND ACINI - A MORPHOLOGICAL-STUDY, Radiation research, 140(3), 1994, pp. 419-428
Citations number
50
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Radiology,Nuclear Medicine & Medical Imaging
Journal title
ISSN journal
0033-7587
Volume
140
Issue
3
Year of publication
1994
Pages
419 - 428
Database
ISI
SICI code
0033-7587(1994)140:3<419:TROSGI>2.0.ZU;2-B
Abstract
The aim of this study was to investigate the radiosensitivity of saliv ary gland tissue pretreated with isoproterenol to establish a status o f depletion of secretory granules in acinar cells at the time of irrad iation. Nuclear aberrations and cell lysis were taken as parameters fo r cell death. Local X irradiation with a single dose of 15 Gy induced comparable early morphological changes in the rat parotid and submandi bular glands. During the first day after irradiation, the most obvious changes were degranulation of serous cells and induction of nuclear a berrations in both the secretory (serous as well as mucous) and interc alated duct compartment. Subsequently, progressive lysis occurred in s ecretory units but not in intercalated and striated ducts. Recovery of tissue integrity was observed from day 6. Early radiation-induced cel l death was not reduced by isoproterenol-induced degranulation of acin ar cells before irradiation. Subsequent recovery from radiation damage seemed to occur earlier in parotid glands but not in submandibular gl ands pretreated with isoproterenol. From the present study it is concl uded that the radiosensitivity of serous salivary gland acini is not d ependent on the presence of secretory granules at the time of irradiat ion. There was some evidence for a faster recovery from radiation dama ge observed after pretreatment with isoproterenol which may be the res ult of drug-induced stimulation of cell proliferation.