HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION USED FOR LABORATORY ROUTINE DETERMINATION OF FLUVOXAMINE IN HUMAN PLASMA

Citation
A. Belmadani et al., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION USED FOR LABORATORY ROUTINE DETERMINATION OF FLUVOXAMINE IN HUMAN PLASMA, Human & experimental toxicology, 14(1), 1995, pp. 34-37
Citations number
14
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Toxicology
ISSN journal
0960-3271
Volume
14
Issue
1
Year of publication
1995
Pages
34 - 37
Database
ISI
SICI code
0960-3271(1995)14:1<34:HLWUDU>2.0.ZU;2-9
Abstract
Fluvoxamine is an antidepressant drug introduced into the clinic in 19 86. It acts by selectively inhibiting neuronal serotonin recapture. It can be quantified by several methods, including high performance liqu id chromatography. The HPLC method used so far needs special equipment and has poor sensitivity. The technique is difficult and time consumi ng. An easier, quicker and more sensitive HPLC assay for the routine d etermination of fluvoxamine in human plasma has therefore been develop ed. After alkalinisation and direct extraction by a mixture of n-hexan e-isoamylic alcohol 985: 15 (v/v) of plasma samples, the organic phase s were further extracted by HCl 0.1 N. Thirty mu L of the final extrac t (with loxapine as internal standard) were injected directly into a C -8 column with a mobile phase consisting of 370 mt acetonitrile, 0.4 m t diethylamine, 630 mt of distilled water, 25 mt pic B5. UV detection at 254 nm was used. The whole process was completed in 40 min. The det ection limit was 10 ng mL(-1). No interference was found either with s everal benzodiazepines or with antidepressant drugs commonly associate d during treatments.