P2 PROTEIN ENCODED BY GENOME SEGMENT S2 OF RICE DWARF PHYTOREOVIRUS IS ESSENTIAL FOR VIRUS-INFECTION

Citation
J. Yan et al., P2 PROTEIN ENCODED BY GENOME SEGMENT S2 OF RICE DWARF PHYTOREOVIRUS IS ESSENTIAL FOR VIRUS-INFECTION, Virology, 224(2), 1996, pp. 539-541
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Virology
Journal title
ISSN journal
0042-6822
Volume
224
Issue
2
Year of publication
1996
Pages
539 - 541
Database
ISI
SICI code
0042-6822(1996)224:2<539:PPEBGS>2.0.ZU;2-J
Abstract
Loss of infectivity to insect vector cell monolayers of rice dwarf phy toreovirus (RDV) after CCl4 treatment was associated with the removal of one of the viral proteins from the virus particles. This protein, e ncoded by genome segment S2 and thus named P2 protein, was located at the outer capsid of the virus particle. When RDV was treated with CCl4 for various times, the reduction in the amount of P2 protein and the loss of viral infectivity to vector cell monolayers were proportional to treatment time. RDV purified using CCl4 treatment, thus lacking P2 protein, lost the ability to infect vector insects through feeding and consequently failed to be transmitted to plants. These results sugges t that P2 protein is essential for RDV infection of the insect cells a nd may be related to transmission of the virus by the vector insect. ( C) 1996 Academic Press, Inc.