INTERACTIONS BETWEEN STAT AND NON-STAT PROTEINS IN THE INTERFERON-STIMULATED GENE FACTOR-3 TRANSCRIPTION COMPLEX

Citation
Cm. Horvath et al., INTERACTIONS BETWEEN STAT AND NON-STAT PROTEINS IN THE INTERFERON-STIMULATED GENE FACTOR-3 TRANSCRIPTION COMPLEX, Molecular and cellular biology, 16(12), 1996, pp. 6957-6964
Citations number
46
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,"Cell Biology
ISSN journal
0270-7306
Volume
16
Issue
12
Year of publication
1996
Pages
6957 - 6964
Database
ISI
SICI code
0270-7306(1996)16:12<6957:IBSANP>2.0.ZU;2-Q
Abstract
The first STAT-containing transcription factor to be studied, the alph a-interferon-induced ISGF3, is composed of a Stat1:2 heterodimer and a weak DNA-binding protein, p48, that is a member of a growing family o f proteins similar to the so-called interferon regulatory factor (IRF- 1). The p48 and Stat1:2 heterodimer do not associate stably in the abs ence of DNA, but we show that amino acids similar to 150 to 250 of Sta t1 and a COOH-terminal portion of p48 exhibit physical interaction, im plying contact that stabilizes ISGF3. Moreover, amino acid exchanges w ithin the Stat1 contact region diminish or abolish the functional acti vity of Stat1. This protein interaction domain may be important in oth er STAT proteins to recruit partners to multiprotein transcription fac tors.