PHYLOGENETIC DIVERSITY WITHIN THE GENUS NITROSOMONAS

Citation
A. Pommereningroser et al., PHYLOGENETIC DIVERSITY WITHIN THE GENUS NITROSOMONAS, Systematic and applied microbiology, 19(3), 1996, pp. 344-351
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
0723-2020
Volume
19
Issue
3
Year of publication
1996
Pages
344 - 351
Database
ISI
SICI code
0723-2020(1996)19:3<344:PDWTGN>2.0.ZU;2-Y
Abstract
The phylogenetic relationships within the genus Nitrosomonas were exam ined in order to obtain a basis for the development of a taxonomically , as well as ecophysiologically related gene probe system suitable for in situ analysis of natural populations of ammonia-oxidizing bacteria . In addition to the 10 validly described species and three undefined species of the genus Nitrosomonas, Nitrosococcus mobilis was included in this study. On the basis of 16S rRNA gene sequence results and DNA- DNA hybridization data, six distinct lines of descent, containing clos ely related or single species, can be distinguished. One of these line ages allied N. europaea and N. eutropha, which are both common in eutr ophic freshwater habitats and which are both halotolerant, together wi th the obligately halophilic N. halophila. FLU these three species are urease negative. A second lineage vr;as defined by the three marine s pecies N. marina, N. aestuarii and Nitrosomonas sp, Nm 51, which are a ll obligately halophilic and urease positive. Two other distinct group s comprised oligotrophic Nitrosomonas species originating from terrest rial or freshwater environments. Members of one group were N. ureae an d N. oligotropha, both being urease positive, and the other group cont ained the urease positive N. nitrosa together with the urease negative N. communis and Nitrosomonas spp. Nm 33 and Nm 41. The remaining two lineages were represented by single marine species, N. cryotolerans an d Nitrosococcus mobilis, respectively. Based on currently available 16 S rDNA sequences primer pairs useful fur specific PCR assisted detecti on of ecophysiologically defined groups of ammonia oxidizers were sele cted.